Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova DNA Suspension Buffer, pH 8.0. 100mL, Sterile. DNase/RNase Tested, PCR Grade.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Medchemexpress LLC MES potassium | 39946-25-3 | 99.88% | 100 G
MES (2-Morpholinoethanesulphonic acid) potassium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer and is widely used in biochemistry and molecular biology experiments such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments (e.g., cell culture, enzyme activity determination, electrophoresis, protein studies)
- Zwitterionic buffer, provides effective buffering capacity around pH 6.15
- Pka value changes with temperature, requiring consideration for different temperatures
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions (e.g., Mg2+, Ca2+, Mn2+), suitable for studies involving metal ions
Medchemexpress LLC MES sodium salt (2-Morpholinoethanesulphonic acid sodium salt) | 71119-23-8 | 98.0% | 1 ML
MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging from pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity around pH 6.15
- Poor affinity with metal ions
- Suitable for studies involving metal ions
Quality Biological Inc Tris HCl (2 M) pH 6.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt | 103404-87-1 | | 25g
HEPES hemisodium salt | Purity: 99% | MW: 249.3 | 103404-87-1 | 25g
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | C29H33N3O9S2 | 10 MG
AR-13324 analog mesylate is a research-grade mesylate salt analog of AR-13324, a Rho-associated kinase (ROCK) and norepinephrine transporter (NET) inhibitor. It is supplied as a light brown to brown liquid intended for laboratory research and assay development.
- Research-use compound related to ROCK and NET inhibition.
- High purity (98.0%) suitable for analytical and biological assays.
- Molecular weight 631.72 g/mol for formulation calculations.
- Supplied as a mesylate salt in a liquid form for easy handling.
- Store sealed, away from moisture; in solvent: -80°C for 6 months, -20°C for 1 month.
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 25g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 25g
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 100g
BIS-TRIS | Purity: ≥99.0% (NT) | MW:209.24 | 6976-37-0 | MFCD00002853 | 100g
Teknova 1M Tris-HCl, pH 8.0 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 2X HBS (HEPES-Buffered Saline), pH 7.05. 250mL, Endotoxin Tested. Sterile. 4 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1X PBS with 0.05% Tween 20, 0.1% BSA. 1000mL, Sterile. 8 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Quality Biological Inc TRIS HCL (1 M), pH 7.0, 500 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ Provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels.¿ Use Tris-HCl 1 M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ pH: 7.5¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
AG Scientific Inc PBS Tablets 100 Tablets
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L