Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc Tris HCl 1 kg
Short for Tris (hydroxymethyl) aminomethane (THAM) hydrochloride, Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Biotang Inc TRIS-HEPES-Native Running Buffer (10X), 500 ml
TRIS-HEPES-Native Running Buffer (10X), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.6 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.6 (0.2 micron filtered), 500 ml.
![Alkali Scientific bis TRIS [bis (2 hydroxyethyl)aminotris(hydroxymethyl)methane], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659835.jpg-250.jpg)
Alkali Scientific bis TRIS [bis (2 hydroxyethyl)aminotris(hydroxymethyl)methane], 1 Kg
Bis TRIS (bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane) is available in a high-purity 1 kg powder form, ideal for high-volume laboratories and research applications requiring precise pH control in the alkaline range. This buffer is commonly used in protein electrophoresis, enzyme kinetics studies, and biochemical analysis. Bis TRIS offers excellent buffering capacity at pH values above 7.0, making it particularly useful in applications where pH stability is essential. The 1 kg package is a cost-effective option for laboratories conducting large-scale experiments, offering reliable performance for extended periods. Bis TRIS is frequently used in academic, clinical, and pharmaceutical research settings where exact pH control is critical for experimental accuracy.
Biotang Inc BioAcryl-P (30%) Bis-Acrylamide 39:1, 1000 ml
BioAcryl-P (30%) Bis-Acrylamide 39:1, 1000 ml.
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 1Kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
AdipoGen Trishydroxymethyl aminometha
Chemical. CAS 77-86-1. Formula C4H11NO3. MW 121.14. Synthetic. A high quality buffer used in the range of physiological pH 7-9. Widely used as a biological buffer or as a component of buffer formulations. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms pH 7.0-9.0. Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
Bioworld PEB- Protein Extraction Buffer (5x), 500 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Teknova 10mM Tris-HCl, pH 8.5. 2mL, 100-Pack, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.